Mouse Anti-Human PINK1 Monoclonal IgG1, Clone S4-15

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Size

100 µg

Catalog no

SMC-450D-FITC
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Representative figure legend

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (SMC-450). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (SMC-450) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PINK1 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~50 kDa PINK1 protein using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (SMC-450). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (SMC-450) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa. Mouse Anti-PINK1 Antibody [S4-15] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-450) Mouse Anti-PINK1 Antibody [S4-15] used in Western Blot (WB) on Rat Brain (SMC-450)

Scientific context

PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports; PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Representative figure link

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Antibody's other name

Parkinson disease (autosomal recessive) 6 Antibody, EC 2.7.11.1 Antibody, PARK6 Antibody, serine/threonine-protein kinase PINK1 mitochondrial Antibody, PTEN-induced putative kinase protein 1 Antibody, protein kinase BRPK Antibody, BRPK Antibody, FLJ27236 Antibody, PTEN induced putative kinase 1 Antibody, Phosphatase and Tensin Homolog Antibody

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Antibody certificate of analysis

1 µg/ml of SMC-450 was sufficient for detection of PINK1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Immunogen

Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. 82% identical to rat and 81% identical to mouse.>30% identity with DMPK.

Primary research fields

Neuroscience, Cell Signaling, Post-translational Modifications, Phosphorylation, cardiovascular System, Heart

Antibody's dilutions

WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody's full description

Mouse Anti-Human PINK1 Monoclonal IgG1 Antibody, Clone: S4-15: FITC

Antibody in cell

Mitochondrion , Mitochondrion Outer Membrane , Cytoplasm

Tissue specificity

See included datasheet or contact our support service

Antibody buffer for storage

PBS pH 7.4, 50% glycerol, 0.1% sodium azide

Antibody's datasheet

Contact our support service

Antibody's category

Monoclonal Antibodies

Purity

Protein G Purified

Antibody's reactivity

Human, Mouse, Rat

Shipping recommendations

Blue Ice or 4ºC

Antibodies' applications

WB, IHC, ICC/IF

Antibody's specificity

Detects ~50kDa.

Warning information

Non-hazardous

Latin name

Mus musculus

Tested reactivity

To be tested

Tested applications

To be tested

NCBI number

NP_115785.1

Released date

26-Sep-2013

Protein number

Q9BXM7.1

Stock availability

In Stock

Antibody's concentration

1 mg/ml

Storage recommendations

-20ºC

Country of production

Canada

Raised in

Mouse

Antibody's target

PINK1

Clone

S4-15

Gene number

65018

Brandname

None

Additional isotype

IgG1

Net weight (g)

0.1

Total weight (kg)

1.4

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