Mouse Melanocyte-stimulating hormone receptor (MC1R) ELISA Kit, 96 well plate

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Size

96T

Catalog no

EKC37374
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Description

Hormone releasing factors and releasing hormones are  signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Warnings

The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternate gene name

CMM5, MGC14337, MSH-R, SHEP2, melanocortin 1 receptor|melanotropin receptor

Gene name

melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)

ELISA's specificity

To know the specificity of this test please feel free to contact us

QC

The kit is manufactured at ISO 9001 certified facilities.

Notes

For research use only. Not for diagnostic procedures.

ELISA's stability

Please see ELISA's datasheet, otherwise contact us

Assay principle

Please see ELISA's datasheet, otherwise contact us

Protocol

Please see ELISA's datasheet, otherwise contact us

Precision of the test

Please see ELISA's datasheet, otherwise contact us

Samples to be analyzed

serum, plasma, tissue homogenates, cell lysates

Alternate protein number

Please refer to SwissProt

Gene number

Please refer to GenBank

Detection limits

31.25 pg/ml-2000 pg/ml

Estimated production time

7-11 business days

Assay class

Please contact us

Research main area

Neuroscience

Latin name

Mus musculus

ELISA detection

Colorimetric

Assay duration

1-5 hours

Sensitivity limit

7,8 pg/ml

Use for

6 months

Shipping requirements

Blue ice

Protein number

Q01727

Verified applications

ELISA

Verified reactivity

Mouse

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