Mouse αMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit

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Size

96 Tests

Catalog no

EKE61894
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Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse αMSH . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse αMSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse αMSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse αMSH. The concentration of Mouse αMSH in samples can be calculated by comparing the OD of the samples to the standard curve.

Description

The Mouse αMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.Hormone releasing factors and releasing hormones are  signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Shelf life

Use Mouse αMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit before 6 months

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Cross Activity

No significant cross-reactivity or interference was observed.

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Research Area

Cancer, Metabolism, Neuroscience, Signal transduction

Application

For research use only. Not for diagnostic procedures.

Storage Temperature

Short term: 4°C; Long term: see manual.

Sample Type

Serum, Plasma, Biological Fluids

Estimated Turnaround Time

6-11 business days

Detection Range

15.63-1000 pg/mL

Detection Method

Colorimetric

Latin name

Mus musculus

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice packs

Assay Time

4.5 hours

Assay Type

Sandwich

Species Reactivity

Mouse

Target's alterntive name

αMSH

Target Name

NA

Specificity

NA

Stability

NA

Precision

NA

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