LAMC2 Antibody

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Size

0,1 mg

Catalog no

PA1582
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Background

Laminin gamma2, Laminin subunit gamma-2, is a protein that in humans is encoded by the LAMC2 gene. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma(formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly though to be a truncated version of beta chain(B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5(earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized.

Related articles

1. Aberdam, D., Galliano, M.-F., Vailly, J., Pulkkinen, L., Bonifas, J., Christiano, A. M., Tryggvason, K., Uitto, J., Epstein, E. H., Jr., Ortonne, J.-P., Meneguzzi, G.Herlitz's junctional epidermolysis bullosa is linked to mutations in the gene (LAMC2) for the gamma-2 subunit of nicein/kalinin (laminin-5).Nature Genet. 6: 299-304, 1994. 2. Airenne, T., Haakana, H., Sainio, K., Kallunki, T., Kallunki, P., Sariola, H., Tryggvason, K.Structure of the human laminin gamma-2 chain gene (LAMC2): alternative splicing with different tissue distribution of two transcripts.Genomics 32: 54-64, 1996. 3. Baudoin, C., Miquel, C., Gagnoux-Palacios, L., Pulkkinen, L., Christiano, A. M., Uitto, J., Tadini, G., Ortonne, J.-P., Meneguzzi, G.A novel homozygous nonsense mutation in the LAMC2 gene in patients with the Herlitz junctional epidermolysis bullosa.Hum. Molec. Genet. 3: 1909-1910, 1994.

Synonyms

3918 antibody|B2T antibody|BM600 antibody|Cell-scattering factor 140 kDa subunit antibody|CSF 140 kDa subunit antibody|CSF antibody|EBR2 antibody|EBR2A antibody|Epiligrin subunit gamma antibody|Kalinin subunit gamma antibody|Kalinin/nicein/epiligrin 100 kDa subunit antibody|Ladsin 140 kDa subunit antibody|LAMB2T antibody|LAMC2 antibody|LAMC2_HUMAN antibody|Laminin 5 gamma 2 subunit antibody|Laminin B2t chain antibody|Laminin gamma 2 antibody|laminin gamma 2 chain antibody|Laminin subunit gamma-2 antibody|Laminin-5 subunit gamma antibody|LAMNB2 antibody|Large adhesive scatter factor 140 kDa subunit antibody|MGC138491 antibody|MGC141938 antibody|Nicein subunit gamma antibody|NICEIN-100KDA antibody

Storage condtions

Keep the LAMC2 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.

Tips

The LAMC2 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LAMC2(1160-1180aa ETSIDGILADVKNLENIRDNL), identical to the related rat and mouse sequences.

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Solubilization

The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml

Product configuration

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification

Immunogen affinity purified.

Protein Name

Laminin subunit gamma-2

Type of the antibody

IgG polyclonal antibody

Clone

Polyclonal antibody

Clonality

Polyclonal antibody

Gene Full Name

laminin, gamma 2

Product form

freeze-dried

French translation

anticorps

Analyses

WB,IHC-P

Uniprot ID

Q13753

Raised in

rabbit

Gene Name

LAMC2

Reacts with species:

human

Target antigen

LAMC2

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