Laminin gamma 1 Antibody

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Size

0.1mg

Catalog no

R30658
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Description

Laminin subunit gamma-1 is a protein that in humans is encoded by the LAMC1 gene. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery. The biological functions of the different chains and trimer molecules are largely unknown, but some have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform. The gamma 1 chain, formerly though to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes.

Storage

After reconstitution, the Laminin gamma 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Notes

The stated application concentrations are suggested starting points. Titration of the Laminin gamma 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Immunogen

Amino acids 1566-1579 (KKQEAAIMDYNRDI-human) were used as the immunogen for this Laminin gamma 1 antibody.

Intented use

This Laminin gamma 1 antibodyis to be used only for research purposes and not for diagnostics..

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Recommended dilutions

Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml

Host animal

Rabbit (Oryctolagus cuniculus)

Clonality

Polyclonal (rabbit origin)

Form

Antigen affinity purified

Clone

Polyclonal antibody

Purity

Antigen affinity

Recognised antigen

Laminin gamma 1

Conjugation

Unconjugated

French translation

anticorps

Tested applications

WB, IHC-P

Category

Antibody

Uniprot #

P11047

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