LAMC2 Antibody

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Size

0.1mg

Catalog no

R30659
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Description

Laminin subunit gamma-2 is a protein that in humans is encoded by the LAMC2 gene. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. This gene encodes the gamma chain isoform laminin, gamma2. The gamma2 chain, formerly though to be a truncated version of beta chain(B2t), is highly homologous to the gamma1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma1, and is specifically localized to epithelial cells in skin, lung and kidney. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized.

Storage

After reconstitution, the LAMC2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Notes

The stated application concentrations are suggested starting amounts. Titration of the LAMC2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of LAMC2 (ETSIDGILADVKNLENIRDNL) was used as the immunogen for this LAMC2 antibody (100% homologous in human, mouse and rat).

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Intented use

This LAMC2 antibodyis to be used only for research purposes and not for diagnostics..

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Recommended dilutions

Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml

Host animal

Rabbit (Oryctolagus cuniculus)

Clonality

Polyclonal (rabbit origin)

Form

Antigen affinity purified

Clone

Polyclonal antibody

Purity

Antigen affinity

Conjugation

Unconjugated

French translation

anticorps

Tested applications

WB, IHC-P

Category

Antibody

Uniprot #

Q13753

Recognised antigen

LAMC2

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