anti-SMC1 (phospho Ser957) Antibody

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Size

0.1 mg

Catalog no

79-193
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Gene info

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Purity

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Antigen

SMC1 (Phospho-Ser957) antibody was made against a peptide sequence around phosphorylation site of serine 957 (G-S-S (p) -Q-G) derived from Human SMC1.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Suspention, pH, azide

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Gene names

SMC1, SMCB, CDLS2, SB1.8, SMC1L1, DXS423E, SMC1alpha, APRF, SM1A, SMC1A, SMC1 alpha protein

Spicific to

This antibody detects endogenous level of SMC1 only when phosphorylated at serine 957.

Antibody advice

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Keep at

Store antibody at -20 grades C for up to one year.

Antigene name

structural maintenance of chromosomes 1A

Immuno application

Western Blot, immuno-histo-chemistry

Antibodie's label

unlabelled serum

Works with

Human, Mouse

Source

Homo sapiens

Ncbi nr

NP_006297.2

Vial with antibody

in solution

Type

Polyclonal

French translation

anticorps

Omim nr

30581135

Quantity per ml

1 mg/mL

KDa

145 kDa

Protein nr

Q14683

Ab raised in

Rabbit

GENE symbol

SMC1A

NCBI GENE nr

8243

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