Lenti kit or vector with CMV or EF promotors for producing Lentivirus (lenti-, Latin for "slow") or lentiviral particles in 293t cells. Lentiviruses are characterized by a long incubation period and often Puro selected. Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells. They are one of the most efficient methods of a gene delivery vector. The are packaged in HEK 293T cells to get the highest possible lentiviral titers of 108 IFU/ml. The Luc reporter is the expression of the firefly luciferase gene. Puro stands for puromicin selection.