Mouse γMSH(gamma-Melanocyte Stimulating Hormone) EIA Kit

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Size

96Test

Catalog no

E-EL-M2636-96T
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Test principle

This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse γMSH. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse γMSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse γMSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse γMSH. You can calculate the concentration of Mouse γMSH in the samples by comparing the OD of the samples to the standard curve.

Description

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Hormone releasing factors and releasing hormones are  signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay

Product Name

Mouse γMSH(gamma-Melanocyte Stimulating Hormone) EIA Kit

Tested Sample Types

Serum, Plasma, Cell supernatant

Detection Range

15.625~1000pg/mL

Latin name

Mus musculus

Detection Type

Colormetric

Sensitivity

9.38 pg/mL

Type

Sandwich

Target Species

Mouse

Target Name

γMSH

Target Synonym

NA

UNIProt ID

NA

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