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Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Western blot analysis of extract of various cells, using DMC1 antibody.
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
DMC1 polyclonal antibody detects endogenous levels of DMC1 protein.
For research use only, not for use in diagnostic procedure.
Recombinant protein of human DMC1.
WB 1:500 - 1:2000 IP 1:50 - 1:200
Polyclonals and antibodies
DMC1H, LIM15
Human,Mouse
anticorps
~ 38 kDa
Q14565
WB IP