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Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Western blot (WB) analysis of NPY5-R (M264) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Neuropeptide Y receptor type 5; NPY5-R; NPY-Y5 receptor; NPYY5-R; Y5 receptor; NPY5R; NPYR5
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
NPY5-R (M264) polyclonal antibody detects endogenous levels of NPY5-R protein.
Synthetic peptide, corresponding to amino acids 230-286 of Human NPY5-R.
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
For research use only, not for use in diagnostic procedure.
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Polyclonals and antibodies
Human,Mouse,Rat
WB IHC IF
anticorps
~ 48 kDa
Q15761