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Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Western blot (WB) analysis of MC1-R (V312) polyclonal antibody at 1:1000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:The Brain tissue lysate of Mouse(40ug)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Melanocyte-stimulating hormone receptor; MSH-R; Melanocortin receptor 1; MC1-R; MC1R; MSHR
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
MC1-R (V312) polyclonal antibody detects endogenous levels of MC1-R protein.
Synthetic peptide, corresponding to amino acids 300-350 of Human MC1-R.
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
For research use only, not for use in diagnostic procedure.
WB: 1:500~1:1000 IF: 1:50~1:200
Polyclonals and antibodies
Human,Mouse,Rat
anticorps
~ 35 kDa
Q01726
WB IF