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Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Western blot (WB) analysis of Bax (S4) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Bax (S4) polyclonal antibody detects endogenous levels of Bax protein.
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX; BCL2L4
Synthetic peptide, corresponding to the N-terminus of Human Bax.
For research use only, not for use in diagnostic procedure.
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
Polyclonals and antibodies
WB IHC IP
anticorps
~ 21 kDa
Q07812
Human